Yes, hell is described in the Bible as eternal separation from God. The Old Testament foreshadows this separation through the consequence Satan faced for his rebellion (Isaiah 14:12–15; Ezekiel 28:12–17). After death, there are only two destinations—heaven for the righteous who have faith in Jesus Christ and hell for the unrighteous who reject Him (Matthew 25:34, 41, 46; Luke 16:22–23). Hell is characterized by outer darkness (Matthew 8:12), a prison (1 Peter 3:19), and a lake of fire (Luke 16:24; Revelation 20:14–15). Second Thessalonians 1:8–9 describes it as eternal punishment, entirely separated from the Lord’s presence. This is what those who reject Jesus reveal that they desire, but they do not realize what they are asking for. To be apart from God is to be separated from all that is good because He is the source of all that is good (James 1:17). However, Jesus Himself bore our punishment for sins, so all who put their faith in Him as Savior will receive His forgiveness and eternal life.
There will be no second chances to repent and be saved after death (Hebrews 9:27; Jude 1:13; Matthew 25:46). Hell has no redemption to offer, only suffering. The distinction between light and darkness, good and evil, will be unmistakable. To be in hell is to be apart from God's light (1 John 1:5), peace (Ephesians 2:14), mercy (Lamentations 3:23–24), joy (Matthew 25:23), and love (1 John 4:8). To be apart from God is to be separated from all that is good because He is the source of all that is good (James 1:17).
Jesus Himself bore our punishment for sin, but until we put our faith in Him as our Savior, we will not receive His forgiveness and eternal life: "Believe in the Lord Jesus, and you will be saved" (Acts 16:31). In Jesus, we are no longer condemned but forgiven: "There is therefore now no condemnation for those who are in Christ Jesus" (Romans 8:1). Instead of being condemned for our sins and sent to an eternity in hell, Jesus's sacrifice gives us the ability to have everlasting life with Him in heaven (John 3:16–18).
If you have not yet received Jesus as your Savior, and you are interested to know more, please read our article "How can I be saved?"