Yes, the Holy Spirit is present during the tribulation. While 2 Thessalonians 2:7 indicates that the Holy Spirit will not restrain evil during that time, He will still be active in bringing people to salvation, enabling regeneration, and sanctification. The Bible makes it clear that people will be saved during the tribulation (Revelation 7:14; 14:6–7), which confirms the Holy Spirit's ongoing presence and work during that period. This assurance emphasizes God's unwavering commitment to calling people to salvation, even in the most challenging times.
The truth that the Holy Spirit will be present during the tribulation is very encouraging for believers in pre-tribulation times. We often worry deeply about our friends and family who will have to endure the tribulation without Christ. But the Bible promises that a "great multitude" will be saved during that time. Man is drawn to God by the Holy Spirit, born of the Spirit until salvation through faith in Christ, and it is also by the Spirit that we grow and mature in Christ. Without the presence of the Holy Spirit, none of these things would occur during the tribulation. But we know that people will be saved during that time, and we know the Holy Spirit will be at work both to bring people to salvation and to help them grow after salvation. Because of this, we can trust God’s goodness in making Himself known even during the tribulation. We should also be careful to live godly lives and to share the gospel, in an effort to "hold back those who are stumbling to the slaughter" (Proverbs 24:11).